第2生物活性研究部

研究業績

ATP Depletion Assay Led to the Isolation of New 36-Membered Polyol Macrolides Deplelides A and B from Streptomyces sp. MM581-NF15.
T. Takeuchi, M. Hatano, M. Umekita, C. Hayashi, S. Wada, M. Nagayoshi, R. Sawa, Y. Kubota, M. Kawada, M. Igarashi and M. Shibasaki
Org. Lett. 2017, 19, 4207-4210
In vivo efficacy of β-lactam/tripropeptin C in a mouse septicemia model and the mechanism of reverse β-lactam resistance in methicillin-resistant Staphylococcus aureus mediated by tripropeptin C.
H. Hashizume1, Y. Takahashi, T. Masuda, S. Ohba, T. Ohishi, M. Kawada and M. Igarashi
J Antibiot. 2017, Accepted doi: 10.1038/ja.2017.88
Fradiamine A, a new siderophore from the deep-sea actinomycete Streptomyces fradiae MM456M-mF7.
Y. Takehana, M. Umekita, M. Hatano, C. Kato, R. Sawa, and M. Igarashi
J Antibiot. 2017, 70, 611-615
Structures and biological activities of novel 4’-acetylated analogs of chrysomycins A and B.
S. Wada, R. Sawa, F. Iwanami, M. Nagayoshi, Y. Kubota, K. Iijima, C. Hayashi, Y. Shibuya, M. Hatano, M. Igarashi and M. Kawada
J Antibiot. 2017, in press
薬剤耐性と創薬研究
-アミノグリコシド系抗生物質を中心として-
五十嵐 雅之、高橋 良昭
Obe-lisk 2017, 22, 7-12.
Structure and antibacterial activities of new cyclic peptide antibiotics, pargamicins B, C and D, from Amycolatopsis sp. ML1-hF4
H. Hashizume, R. Sawa, K. Yamashita, Y. Nishimura and M. Igarashi.
J Antibiot. 2017, 70, 699-704
Epithelial-mesenchymal transition promotes SOX2 and NANOG expression in bladder cancer.
T. Migita, A. Ueda, T. Ohishi, M. Hatano, H. Seimiya, S. Horiguchi, F. Koga and F. Shibasaki.
Laboratory Investigation, 2017, in press.
Stability and bioavailability of lentztrehaloses A, B, and C as replacements for trehalose.
S. Wada, R. Sawa, S. Ohba, C. Hayashi, M. Umekita, Y. Shibuya, K. Iijima, F. Iwanami and M. Igarashi.
J. Agric. Food Chem. 2016, 64, 7121-7126.
Synthesis and antibacterial activity of tripropeptin C derivatives containing the pyrimidine ring system.
S. Hirosawa, Y. Takahashi, A. Murotani, H. Hashizume, T. Miyake and Y. Akamatsu.
Heterocycles. 2015, 91, 2143-2151.
2014年 浜田賞受賞論文 放線菌を初めとする土壌細菌由来の有用抗菌物質の探索と作用機序解析
橋爪秀樹
日本放線菌学会誌 2015,29,8-12
Novel autophagy inducers lentztrehaloses A, B and C
S. Wada, Y. Kubota, R. Sawa, M. Umekita, M. Hatano, S. Ohba, C. Hayashi, M. Igarashi and A. Nomoto.
J Antibiot. 2015, 68, 521-529
Synthesis and determination of absolute configuration of lentztrehalose A
M. Zhang, S. Wada, F. Amemiya, T. Watanabe and M. Shibasaki
Chem. Pharm. Bull. 2015, in press
Natural lipopeptide antibiotic tripropeptin C revitalizes and synergistically potentiates the activity
of beta-lactams against methicillin-resistant Staphylococcus aureus.
H. Hashizume, Y. Takahashi, S. Harada and A. Nomoto.
J Antibiot. 2015, 68, 373-378
Androprostamines A and B, the new anti-prostate cancer agents produced by Streptomyces sp. MK932-CF8.
Y. Yamazaki, T. Someno, M. Igarashi, N. Kinoshita, M. Hatano, M. Kawada, I.Momose and
A. Nomoto.
J. Antibiot. 2014,68,279-285
Synthesis and antibacterial activity of tripropeptin C derivatives modified at the carboxyl groups.
S. Hirosawa, Y. Takahashi, H. Hashizume, T. Miyake and Y. Akamatsu.
J. Antibiot. 2014, 67, 265-268.
Structure and biological properties of lentztrehalose: a novel trehalose analog.
S. Wada, S. Ohba, T. Someno, M. Hatano and A. Nomoto.
J. Antibiot. 2013, 67, 319-322.
Waldiomycin, a novel WalK-histidine kinase inhibitor from Streptomyces sp. MK844-mF10.
M. Igarashi, T. Watanabe, T. Hashida, M. Umekita, M. Hatano, Y. Yanagida, H. Kino, T. Kimura, N. Kinoshita, K. Inoue, R. Sawa, Y. Nishimura, R. Utsumi and A. Nomoto.
J. Antibiot. 2013, 66, 459-464.
Staphylococcal superantigen-like protein 10 (SSL10) inhibits blood coagulation by binding to prothrombin and factor Xa via their γ-carboxyglutamic acid (Gla) domain.
S. Itoh, R. Yokoyama, G. Kamoshida, T. Fujiwara, H. Okada, T. Takii, T. Tsuji, S. Fujii,
H. Hashizume and K. Onozaki.
J. Biol. Chem. 2013, 288, 21569-21580.
Inhibition of the first step in synthesis of the mycobacterial cell wall core, catalyzed by the GlcNAc-1-phosphate transferase WecA, by the novel caprazamycin derivative CPZEN-45.
Y. Ishizaki, C. Hayashi, K. Inoue, M. Igarashi, Y. Takahashi, V. Pujari, D. C. Crick, P. J. Brennan and A. Nomoto.
J. Biol. Chem. 2013, 288, 30309-30319.
Structure-activity relationship study of intervenolin derivatives:
synthesis, antitumor and anti-Helicobacter Pylori activities.
H. Abe, M. Kawada, H. Inoue, S. Ohba, T. Masuda, C. Hayashi, M. Igarashi,
A. Nomoto, T. Watanabe and M. Shibasaki.
Tetrahedron 2013, 69, 7608-7617.
Intervenolin, a new antitumor compound with anti-Helicobacter pylori activity, from Nocardia sp. ML96-86F2.
M. Kawada, H. Inoue, S. Ohba, M. Hatano, M. Amemiya, C. Hayashi, I. Usami, H. Abe, T. Watanabe, N. Kinoshita, M. Igarashi, T. Masuda, D. Ikeda and A. Nomoto.
J. Antibiot. 2013, 66, 543-548.
Sacchathridine A, a prostaglandin release inhibitor from Saccharothrix sp.
K. Nakae, I. Kurata, F. Kojima, M. Igarashi, M. Hatano, R. Sawa, Y. Kubota,
H. Adachi and A. Nomoto.
J. Natural Products 2013, 76, 720-722.
Vegfrecine, an inhibitor of VEGF receptor tyrosine kinases isolated
from the culture broth of Streptomyces sp.
C. Nosaka, H. Adachi, R. Sawa, K. Nakae, S. Atsumi, N. Kinoshita, Y. Kubota,
M. Igarashi, Y. Sei, K. Yamaguchi, M. Shibuya, Y. Nishimura and Y. Akamatsu.
J. Natural Products 2013, 76, 715-719.
NAD(P)H quinone oxidoreductase 1 (NQO1)-bioactivated pronqodine A
regulates prostaglandin release from human synovial sarcoma cells.
K. Nakae, H. Adachi, R. Sawa, N. Hosokawa, M. Hatano, M. Igarashi,
Y. Nishimura, Y. Akamatsu and A. Nomoto.
J. Natural Products 2013, 76, 510-515.
Novel semisynthetic antibiotics from caprazamycins A-G: caprazene derivatives
and their antibacterial activity.
Y. Takahashi, M. Igarashi, T. Miyake, H. Soutome, K. Ishikawa, Y. Komatsuki,
Y. Koyama, N. Nakagawa, S. Hattori,K. Inoue, N. Doi and Y. Akamatsu.
J. Antibiot. 2013, 66, 171-178.
Amycolamicin: a novel broad-spectrum antibiotic inhibiting bacterial topoisomerase.
R. Sawa, Y. Takahashi, H. Hashizume, K. Sasaki, Y. Ishizaki, M.Umekita, M. Hatano,
H. Abe, T. Watanabe, N. Kinoshita, Y. Homma, C. Hayashi, K. Inoue, S. Ohba,
T. Masuda,  M. Arakawa, Y. Kobayashi, M. Hamada, M. Igarashi, H. Adachi,
Y. Nishimura and Y. Akamatsu.
Chem. Eur. J. 2012, 18, 15772-15781.
Discovery and characterization of NK13650s, naturally occurring
p300-selective histone acetyltransferase inhibitors.
S. Tohyama, A. Tomura, N. Ikeda, M. Hatano, J. Odanaka, Y. Kubota,
M.Umekita, M. Igarashi, R. Sawa and T. Morino.
J. Org. Chem. 2012, 77, 9044-9052.
Curing bacteria of antibiotic resistance: reverse antibiotics, a novel
class of antibiotics in nature.
K. Hiramatsu, M. Igarashi, Y. Morimoto, T. Baba, M. Umekita and Y. Akamatsu.
Int. J. Antimicrob. Agents. 2012, 39, 478-485.
Application of matrix-assisted laser desorption ionization-time of
flight mass spectrometry for discrimination of laboratory-derived
antibiotic-resistant bacteria.
M. Muroi, K. Shima, M. Igarashi, Y. Nakagawa and K. Tanamoto.
Biol. Pharm. Bull. 2012, 35, 1841-1845.
Isolation and characterization of signamycine B, an antibiotic that targets the dimerization domain of histidine kinase WalK.
T. Watanabe, M. Igarashi, T. Okajima, E. Ishii, H. Kino, M. Hatano, R.Sawa,
M. Umekita, T. Kimura, S. Okamoto,Y. Eguchi, Y. Akamatsu and R. Utsumi.
Antimicrob. Agents Chemother. 2012, 56, 3657-3663.
Decalpenic acid induces early osteoblastic markers in pluripotent
mesenchymal cells via activation of retinoic acid receptor gamma.
S. Sakamoto, F. Kojima, I. Momose, M. Kawada, H. Adachi and Y. Nishimura.
Biochem. Biophys. Res. Commun. 2012, 422, 751-757.
抗メチシリン耐性黄色ブドウ球菌薬の開発研究
橋爪秀樹
薬学雑誌 2012, 132, 59-67.
病原性細菌に対する新しい薬剤標的-二成分情報伝達システム
内海龍太郎、五十嵐雅之
薬学雑誌 2012, 132, 51-58.
新しい抗結核薬の開発状況
五十嵐雅之、高橋良昭
日本臨牀:結核とその類縁疾患 2011, 69, 1482-1488.
Tripropeptin C blocks the lipid cycle of cell wall biosynthesis by complex formation with
undecaprenyl pyrophosphate.
H. Hashizume, R. Sawa, S. Harada, M. Igarashi, H. Adachi, Y. Nishimura and
A. Nomoto.
Antimicrob. Agents Chemother. 2011, 55, 3821-3828.
Development of an antivirulence drug against Streptococcus mutans: repression of biofilm formation, acid tolerance, and competence by a histidine kinase inhibitor, Walkmycin C.
Y. Eguchi, N. Kubo, H. Matsunaga, M. Igarashi and R. Utsumi.
Antimicrob. Agents Chemother. 2011, 55, 1475-1484.
Photodegradation of target oligosaccharides by light-activated small molecules.
D. Takahashi, S. Hirono, C. Hayashi, M. Igarashi, Y Nishimura and K. Toshima.
Angew. Chem. Int. Ed. 2010, 49, 10096-10100.
Decalpenic acid, a novel small molecule from Penicillium verruculosum CR37010,
induces early osteoblastic markers in pluripotent mesenchymal cells.
S. Sakamoto, F. Kojima, M. Igarashi, R. Sawa, M. Umekita, Y. Kubota, K. Nakae,
S. Yamaguchi, H. Adachi, Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2010, 63, 703-708.
Isolation and structure elucidation of a novel androgen antagonist, arabilin, produced
by Streptomyces sp. MK756-CF1.
T. Kawamura, T. Fujimaki, N. Hamanaka, K. Torii, H. Kobayashi, Y. Takahashi,
M. Igarashi, N. Kinoshita, Y. Nishimura, E. Tashiro and M. Imoto.
J. Antibiot. 2010, 63, 601-605.
The synthesis of paleic acid, an antimicrobial agent effective against Mannheimia and
Pasteurella, and its structurally related derivatives.
T. Watanabe, I. Kurata, C. Hayashi, M. Igarashi, R. Sawa, Y. Takahashi and
Y. Akamatsu.
Bioorg. Med. Chem. Lett. 2010, 20, 5843-5846.
Biological activities of pargamicin A, a novel cyclic peptide antibiotic from
Amycolatopsis sp.
H. Hashizume, H. Adachi, M. Igarashi, Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2010, 63, 279-283.
Biosynthesis of amycolamicin: the biosynthetic origin of a branched α-aminoethyl
moiety in the unusual sugar amycolose.
S. Tohyama, Y. Takahashi and Y. Akamatsu.
J. Antibiot. 2010, 63, 147-149.
Novel antibacterial compounds specifically targeting the essential WalR response
regulator.
Y. Gotoh, A. Doi, E. Furuta, S. Dubrac, Y. Ishizaki, M. Okada, M. Igarashi, N. Misawa,
H. Yoshikawa, T. Okajima, T. Msadek and R. Utsumi.
J. Antibiot. 2010, 63, 127-134.
Antipain Y, a new antipain analog that inhibits neurotransmitter release from rat
dorsal root ganglion neurons.
K. Nakae, F. Kojima, R. Sawa, Y. Kubota, M. Igarashi, N. Kinoshita, H. Adachi,
Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2010, 63, 41-44.
Identification of the toxic trigger in mushroom poisoning.
M. Matsuura, Y. Saikawa, K. Inui, K. Nakae, M. Igarashi, K. Hashimoto and
M. Nakata.
Nat. Chem. Biol. 2009, 5, 465-467.
High-throughput screening assay of hepatitis C virus helicase inhibitors using
fluorescence-quenching phenomenon.
H. Tani, N. Akimitsu, O. Fujita, Y. Matsuda, R. Miyata, S. Tsuneda, M. Igarashi,
Y. Sekiguchi and N. Noda.
Biochem. Biophys. Res. Commun. 2009, 379, 1054-1059.
Caspase-8 mediates mitochondrial release of pro-apoptotic proteins in a manner
independent of its proteolytic activity in apoptosis induced by the protein synthesis inhibitor acetoxycycloheximide in human leukemia Jurkat cells.
K. Kadohara, M. Nagumo, S. Asami, Y. Tsukumo, H. Sugimoto, M. Igarashi,
K. Nagai and T. Kataoka.
J. Biol. Chem. 2009, 284, 5478-5487.
A new type of tripropeptin with anteiso-branched chain fatty acid from Lysobacter sp.
BMK333-48F3.
H. Hashizume, M. Igarashi, R. Sawa, H. Adachi, Y. Nishimura and Y. Akamatsu.
J. Antibiot. 2008, 61, 577-582.
ERK and p38 MAP kinase are involved in downregulation of cell surface TNF receptor 1
induced by acetoxycycloheximide.
H. Ogura, Y. Tsukumo, H. Sugimoto, M. Igarashi, K. Nagai and T. Kataoka.
Int. Immunopharmacol. 2008, 8, 922-926.
Ectodomain shedding of TNF receptor 1 induced by protein synthesis inhibitors regulates
TNF-α-mediated activation of NF-κB and caspase-8.
H. Ogura, Y. Tsukumo, H. Sugimoto, M. Igarashi, K. Nagai and T. Kataoka.
Exp. Cell Res. 2008, 314, 1406-1414.
Discovery of incednine as a potent modulator of the anti-apoptotic function of Bcl-xL from microbial origin.
Y. Futamura, R. Sawa, Y. Umezawa, M. Igarashi, H. Nakamura, K. Hasegawa,
M. Yamasaki, E. Tashiro, Y. Takahashi, Y. Akamatsu and M. Imoto.
J. Am. Chem. Soc. 2008, 130, 1822-1823.