1. Toxicity and PK/PD studies

①Acute toxicity

• Weight changes
• External changes
• Anatomy findings
  • Observation for 14 days

②Pharmacokinetic examination

• Serum
• Urine
• Excrement

2. Antitumor studies

①Antitumor examination

Human transplantable tumor

• Lung : LX-1, DMS-273
• Pancreatic : Capan-1, PANC-1
• Colon : DLD-1
• Prostate : LNCaP, PC-3, DU-145
• Breast : BSY-1
• Melanoma : LOX
• Others

DMS-273 : Small cell lung carcinoma view

Human pancreatic carcinoma view

Mouse transplantable tumor

• Ehrlich carcinoma
• IMC carcinoma
• Sarcoma : S180
• Fibro sarcoma : Meth-A
• Leukemia : L1210, EL4
• Colon cancer : Colon26
• Melanoma : B16-F10, B16-BL6
• Others

②Antiangiogenic examination

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③Antimetastatic examination

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3. Antibacteria study

Model of the systemic infection caused by Staphylococcus aureus Smith.

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4. Antiarthritic study

To induce arthritis, type II collagen emulsified with complete Freund’s adjuvant (CFA) is inoculated into the mouse on days 0 and 21. Footpad volumes and joint changes in the limbs are measured.

The experiment is performed in accordance with the Guidelines for Animal Experiments in the MCRF.